MF Researcher Database

Researchers Database

Advance

Tsumoto Hiroki

    Proteome Research　Senior Researcher
Last Updated :2024/12/27

Researcher Information

URL

J-Global ID

Research Areas

  • Life sciences / Pharmaceuticals - analytical and physicochemistry

Published Papers

MISC

Industrial Property Rights

Research Grants & Projects

  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2023/04 -2026/03 
    Author : 津元 裕樹
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2019/04 -2022/03 
    Author : Tsumoto Hiroki
     
    In this study, a method for analysis of sialyl glycans and glycopeptides using sialic acid linkage-specific derivatization was developed to discriminate between alpha2,3- and alpha2,6-sialic acid. Semi-supercentenarians (SSCs, older than 105 years old) are a good example of human longevity, therefore physiological and biochemical analyses of SSCs can contribute to the understanding of healthy human aging. Using a combination of the developed method and LC-MS/MS, N-glycomics and N-glycoproteomics of plasma proteins obtained from SSCs and younger control groups were performed. As a result of LC-MS/MS analyses, 1676 N-glycopeptides from 91 proteins were identified and quantified. Among them, 366 N-glycopeptides were altered in SSCs compared to younger control groups. Furthermore, multivariate analysis identified 10 N-glycopeptides which were characteristic of SSCs. Most of the identified N-glycopeptides were derived from alpha1-acid glycoprotein 1.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
    Date (from‐to) : 2017/06 -2019/03 
    Author : Iwamoto Machiko
     
    Diabetes mellitus (DM) is known to elevate the risk of Alzheimer’s disease (AD), and AD causes to deteriorate prognosis of DM. Therefore, the diagnostic biomarkers of cognitive impairment at early stage is required for early intervention to prevent AD in DM patients. In the present study, we performed comparative analysis of plasma proteome profiles before and after onset of cognitive impairment in each subject using longitudinal cohort (SONIC), and found the candidate proteins of diagnostic biomarkers of cognitive impairment at early stage. In future study, we will perform validation study for these candidates.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2016/04 -2019/03 
    Author : Miura Yuri; TSUMOTO hiroki; HASHII noritaka; ENDO tamao
     
    We performed glycoproteomics using plasma of semi-super centenarians (SSCs, older than 105 years) and identified core proteins conjugated multi-branched and highly sialylated N-glycans that are characteristic of extreme human longevity. We found that tri-antennary and sialylated N-glycans of haptoglobin characterised SSCs. Furthermore, combining glycomics with derivatization termed sialic acid linkage specific alkylamidation (SALSA), we suggested that sialic acid linkage was likely to change in N-glycans of plasma proteins in SSCs.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2016/04 -2019/03 
    Author : Tsumoto Hiroki
     
    In this study, a method for the enrichment of O-GlcNAcylated peptides using two-step click chemistry was developed. Analysis of O-GlcNAcylated proteins was performed using extracts from rat kidney tissue. A total of 34 O-GlcNAcylated peptides from 28 proteins were identified. Of these, 2 proteins were increased and 7 proteins were decreased in diabetic rat.
  • シアル酸結合様式を区別したN結合型糖鎖解析による健康長寿マーカーの開発
    三井住友海上福祉財団：2016年度 研究助成（高齢者福祉部門）
    Date (from‐to) : 2016/11 -2017/11 
    Author : 津元 裕樹
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2014/04 -2017/03 
    Author : SAWABE Motoji
     
    Previous studies reported that several proteins produced in vascular smooth muscle cells altered with aging, resulting in the deterioration of vascular function. We performed a proteomic study on the human aortic media with chronological aging, using autopsy samples of the aortic media from elderly, middle-aged, and young subjects. Two proteomic approaches, 2D-DIGE and iTRAQ, were performed. Differentially expressed proteins with aging were categorized into three groups: actin family proteins, extracellular matrix proteins, and antioxidant proteins. A decrease in actin and increase in other actin-related proteins in elderly media were found. The immunohistochemical analysis showed that expressions of antioxidant proteins and oxidation stress-marker proteins were higher in the elderly than in the young. In conclusions, the imbalance between actin and actin-related proteins occur with human aortic aging, and antioxidant proteins were induced by elevations in oxidative stress with aging.
  • 糖尿病におけるO-GlcNAc化修飾異常の解析
    花王健康科学研究会：第13回花王健康科学研究助成金
    Date (from‐to) : 2015/12 -2016/11 
    Author : 津元 裕樹
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
    Date (from‐to) : 2013/04 -2015/03 
    Author : TSUMOTO HIROKI
     
    Protein S-nitrosylation is an important post-translational modification in a wide range of biological processes. However, direct detection of S-nitrosylated peptide using mass spectrometry is difficult because of instability and low abundance. The aim of this study is to develop a specific and sensitive method for analysis of protein S-nitrosylation using mass spectrometry. It was demonstrated that triphenylphosphine ester derivative is useful for the selective and efficient reduction of S-NO bonds compared with ascorbate and the water-soluble phosphine TXPTS is useful for the detection of S-nitrosylated peptide using mass spectrometry.
  • 質量分析法を用いた細胞内ニトロキシルの高感度検出法の開発
    科学研究費助成事業 若手研究(B)
    Date (from‐to) : 2010/04 -2012/03 
    Author : 津元 裕樹
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2010 -2012 
    Author : NAKAGAWA Hidehiko; MIYATA Naoki; SUZUKI Takayoshi; TSUMOTO Hiroki
     
    Chemical probes for measuring and imaging nuclei-specific oxidative stress have been synthesized. The probes were fluorescent and responsible to cellular oxidative stress, and one of them was applicable to in vivo-ESR imaging.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
    Date (from‐to) : 2010 -2011 
    Author : TSUMOTO Hiroki
     
    This study aimed to develop specific and highly sensitive method to detect nitroxyl(HNO) by mass spectrometry(MS). A water-soluble thiol compound containing basic moiety to enhance MS signal intensity was synthesized. Sulfinamide produced by the reaction of thiol compound with HNO was detected by multiple reaction monitoring(MRM). Our results suggested that sulfinamide is a useful marker for detection of HNO.

Other link

researchmap


Copyright © MEDIA FUSION Co.,Ltd. All rights reserved.